Human placental metabolism and 'omics'

Maternal exposure to chemicals and drugs during pregnancy will affect the well-being of the mother and newborn child, putatively over generations, by causing modifications at RNA, DNA (genetics /epigenetics) and protein level which may contribute to the developmental origin of diseases. In the present project we’ll study how the above mentioned stress factors affect placental hormonal production (potential for endocrine disruption) and xenobiotics metabolizing characteristics at different stages of pregnancy. Methods used are primary and secondary cell cultures, placental enzymatic determinations in vitro, gene chip and proteomics analysis.

Group leader, Markku Pasanen, Prof.
University of Eastern Finland,
School of Pharmacy, PO Box 1627,
70211 Kuopio, Finland
email: markku.pasanen@uef.fi
tel:+358407199346

Publications:

  • Storvik M, Huuskonen P, Pehkonen P, Pasanen M: The unique characteristics of the transcription of hormonal metabolism enzymes in placenta. Reproductive toxicology, 47:9-14 2014.
  • Huuskonen P, Myllynen P, Storvik M, Pasanen M. The effects of aflatoxin B1 on transporters and steroid metabolising enzymes in JEG-3 cells. Toxicol Lett, 218: 200– 206, 2013.
  • Storvik M, Huuskonen P, Kyllönen T, Lehtonen S, El-NEzami H, Auriola S, Pasanen M: Aflatoxin B1 as an endocrine disrupter up-regulates CYP19A1 in human trophoblastic Jeg-3 cells. Toxicol Letters, 202 161–167, 2011.
  • Obolenskaya M Yu, Teplyuk NN, Sasanova L Ya, Malko MV, Divi RL, Poirier, MC, M Pasanen: Placental glutathione S-transferase activity and polycyclic aromatic hydrocarbon DNA adducts in radioactively and chemically-exposed pregnancies. Toxicology Letters 196: 80-86, 2010.
  • Huuskonen P, Storvik M, Reinisalo M, Honkakoski P, Rysä J, Hakkola J, Pasanen M: Microarray analysis of the global alterations in the gene expression in the placentas from tobacco-smoking mothers. Clinical Pharmacol Ther, 83:542-550, 2008.